General description
The BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell.
Cloning efficiencies are increased 25-1,000 fold relative to other preparations of BL21 cells, which is essential for construction of complex expression libraries.
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Features and Benefits
The unprecedented transformation efficiency of the BL21(DE3) Electrocompetent Cells (> 5 × 109 cfu/μg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment
Components
- BL21(DE3) electrocompetent cells
- pUC 19 transformation control DNA
- recovery medium for expression
biological source: Escherichia coli. grade: for molecular biology. growth mode: adherent or suspension. morphology: rod shaped. technique(s): microbiological culture: suitable. cell transformation: competent cell type: electrocompetent
transformation efficiency: ≥. 5 ×. 109 . cfu/μ. g. shipped in: dry ice. storage temp.: −. 70°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 3.
- UPC:
- 41116111
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- CMC0016-4X25UL