General description
The BL21(DE3) pLysE Chemically Competent Cells produce T7 lysozyme to repress low-level transcription from the T7 promoter prior to IPTG induction, helping to stabilize inserts coding for deleterious products.
These cells have a higher level of repression by T7 lysozyme, offering the greatest control over expression of inserts.
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Features and Benefits
- a transformation efficiency of >1 × 107 cfu/μg.
- economical prices
- offering superior value for everyday protein expression work.
Components
- BL21(DE3) pLysE chemically competent cells
- pUC 19 transformation control DNA
- recovery medium for expression
biological source: Escherichia coli. grade: for molecular biology. growth mode: adherent or suspension. morphology: rod shaped. technique(s): microbiological culture: suitable. cell transformation: competent cell type: chemically competent
transformation efficiency: ≥. 1 x 107 . cfu/μ. g. shipped in: dry ice. storage temp.: −. 70°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 3.
- UPC:
- 41105330
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- CMC0015-20X40UL
