BL21(DE3)PLYSS SINGLES COMPETENT CELLS - NOVAGEN (C15-1307-904)

General description

Genotype: F^-ompT hsdS_B(r_B^- m_B^-) gal dcm (DE3) pLysS (Cam^R)





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BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.

Components

•11 or 22Singles Competent Cells•2 or 4 × 2 mlSOC Medium•10 µlTest Plasmid

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: Escherichia coli. Quality Level: 100. manufacturer/tradename: Novagen^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. growth mode: adherent or suspension. morphology: rod shaped. technique(s): microbiological culture: suitable. cell transformation: transformation efficiency: >. 2×10⁷ . cfu/μ. g. shipped in: dry ice. storage temp.: −. 70°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 2.