Magnetic beads are prepared by covalent immobilization of native protein G (from Streptococcus sp.) to BlackPearl® Magnetic Beads by CNBr activation coupling chemistry. Protein G shows strong binding affinity to Fc region of several classes of mammalian immunoglobulins, BlackPearl® has been used as a reliable tool to purify monoclonal and polyclonal IgGs from ascites, serum and cell culture supernatants. It is an ideal choice for affinity purification and/or fractionation of IgG subclasses which binds poorly to protein A such as bovine, goat, rat, and sheep IgG. These products are supplied as suspension in a buffer containing 100 mM Tris Cl, pH 8.0, 500 mM NaCl, 0.05 % NaN3, and 0.02 % thimerosal. Ligand: Native protein G from Streptococcus sp. Ligand coupling method: CNBr activation Biding capacity: Approximately 20 mg human IgG/ml drained gel Matrix: Magnetic agarose beads, 6% Particle size (range): 50-125 µm Suspension: 25% slurry (1ml contains 250uL settled vol.) pH stability: 3-9 (for use), 2-10 (for cleaning) v Storage: 2-8 °C Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
- UPC:
- 41151513
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 20161003-2
