Bleomycin hydrochloride (C09-1113-442)

Bleomycin hydrochloride is a DNA synthesis inhibitor. Bleomycin hydrochloride is a DNA damaging agent. Bleomycin hydrochloride is an antitumor antibiotic.In VitroBleomycin hydrochloride is chosen as the best-studied micronucleus inducers in human lymphocytes with different mechanisms of genotoxicity. The most frequent Bleomycin-induced DNA lesions are single and double strand breaks and single apuinic/apyrimidinic sites. At the same time Bleomycin is true radiomimetic compound, resembling almost completely the genetic effect of ionizing radiation. ?/nThe IC 50 value of Bleomycin hydrochloride for UT-SCC-19A cell line is 4.0±1.3 nM. UT-SCC-12A and UT-SCC-12B are both more resistant to Bleomycin; IC 50 values are 14.2±2.8 nM and 13.0±1.1 nM, respectively. ?/nBleomycin hydrochloride (50, 100?μM; for 24, 48?h) induce pulmonary fibrosis in RLE-6TN cell (50?μM) and A549 cell (100?μM) . MCE has not independently confirmed the accuracy of these methods. They are for reference only.In VivoBleomycin hydrochloride can be used in animal modeling to construct animal models of pulmonary fibrosis. Bleomycin hydrochloride treatment (3.5-4.0 mg/kg; intra-tracheal) on day 0, body weights decreases by day 4 then increases by Day 7 through the end of the study. Bleomycin hydrochloride (3.5-4.0 mg/kg; intra-tracheal) produces a statistically significant increase in lung hydroxyproline levels, and also increases right caudal lung lobe mass. Bleomycin hydrochloride (intratracheal instillation; 5.0 mg/kg/day) induces pulmonary fibrosis in eighty 8-week-old male BALB/c mice with weight about 20-30 g. Bleomycin induces the expression levels of α-SMA and collagen I. Bleomycin hydrochloride (intratracheally; 2.5 mg/kg; 1.25 mg/ml, approximately 50 μl per mouse) induces pulmonary fibrosis in male C57BL/6 mice (8 weeks old, average weight approximately 24.5 g). MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Male Fischer 344 rats, 8-10 week old, weighing 150-250 gDosage: 3.5-4 mg/kg Administration: Intra-tracheal Result: Body weights decreased by day 4 then increased by Day 7 through the end of the study.Form:Solid. Specification: 0.99 Molecular Formula: C55H85ClN17O21S3+ Molecular Weight: 1452 EC Number: 689-127-6 PubChem CID: 20832750 Isomeric SMILES: CC1=C(N=C(N=C1N)[C@H](CC(=O)N)NC[C@@H](C(=O)N)N)C(=O)N[C@@H]([C@H](C2=CN=CN2)O[C@H]3[C@H]([C@H]([C@@H]([C@@H](O3)CO)O)O)O[C@@H]4[C@H]([C@H]([C@@H]([C@H](O4)CO)O)OC(=O)N)O)C(=O)N[C@H](C)[C@H]([C@H](C)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCCC5=NC(=CS5)C6=NC(=CS6)C(=O)NCCC[S+](C)C)O.Cl