BMPO (C09-1113-226)

BMPO (BocMPO) is a cell-permeable superior spin trap with favorable chemical and spectroscopic features. BMPO (BocMPO) can be used for detecting thiyl radicals, hydroxyl radicals, superoxide anions and glutathiyl radicals.In VitroBMPO is able to trap both •OH and GS• to form more persistent BMPO/•OH and BMPO/•SG adducts. In addition, the electron spin resonance (ESR) spectra of BMPO-derived adducts exhibit high signal-to-noise ratios in biological systems. For example, the ESR spectrum of the BMPO glutathionyl adduct (BMPO/•SG) does not fully overlap with the spectrum of its hydroxyl adduct. The favorable chemical and spectroscopic features make BMPO ideal for the detection of superoxide anions, hydroxyl and thiyl radicals.The potential toxic effects of the spin trap BMPO is measured by two estimates of cell viability (trypan blue exclusion and colony formation).BMPO has no significant effect on cell viability at 2.5 or 25 mM in CHO cells. However, it leads to a significant increase in the number of cells that are unable to exclude Trypan blue at 50 mM BMPO. BMPO significantly reduces a 30% reduction in colony formation at 25 mM concentration in CHO cells. And, BMPO completely inhibits the colony formation of 9L tumor cells at 50 mM.The cell function (rate of oxygen consumption) is also measured, BMPO (25 mM) significantly reduces the oxygen consumption rates in CHO cells.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Form:Solid. Specification: 0.99 Molecular Formula: C10H17NO3 Molecular Weight: 199.25