Bolton Broth Base, 5KG

Bolton Broth Base is recommended for selective enrichment of Campylobacter species from food and animal feeding stuffs. The composition and performance criteria of this medium are as per the specifications laid down in ISO 10272-1 & 2:2017.The media is made selective for Campylobacters by addition of the antibiotics cefoperazone, vancomycin, trimethoprim and amphotericin B. A small quantity of the test sample is inoculated to enrichment medium ratio of 1:10 and homogenized. It is Incubated at 37°C for 4-6 hours, then at 41.5°C for 44 ± 4 hours. Enriched culture is isolated on Karmali Campylobacter Agar Karmali Campylobacter Agar Base (M1222). Appearance: Light yellow to brownish yellow homogeneous free flowing powder Color and Clarity of prepared medium: Basal medium : Brownish yellow colored clear to slightly opalescent solution. After addition of lysed horse blood: Red to brown colored opaque solution in tubes Reaction of 2.76% w/v aqueous solution at 25°C. pH : 7.4±0.2 Cultural characteristics observed with added Bolton Selective Supplement (FD231) after an incubation at 37°C for 5± 1 hours and then at 41.5°C for 44 hours ± 4 hours in a microaerobic atmosphere. Further subculture is carried out on M887I at 41.5°C in a micro-aerobic atmosphere for 44 hours ± 4 hours. For selectivity, further subculture is carried on TSA