BOLTON BROTH SELECTIVE

General description

Bolton Broth Selective Supplement is a mixture of four different antibiotics in lyophilized form. Vancomycin, Cefoperazone, and Trimethoprim inhibit the growth of Gram-positive and Gram-negative bacteria. Amphotericin B largely reduces the growth of Yeasts and Molds.

Application

Bolton Broth Selective Supplement is an additive used during the preparation of Bolton Selective Enrichment Broth for the enrichment of Campylobacter from foods.

Linkage

Replaces: 1.00079.0017

Analysis Note

Growth promotion test according to ISO 11133
Mixed inoculum of Campylobacter, E.coli and P.mirabilis.
Additionaly monoculture of E.coli and P.mirabilis.
Afterwards confirmation on mCCDA resp. Tryptic soy agar
Inoculum on reference medium
- Campylobacter coli ATCC 43478 (WDCM 00004):
10 - 100
- Campylobacter jejuni ATCC 33291 (WDCM 00005): 10 - 100
- Campylobacter jejuni ATCC 29428 (WDCM 00156): 10 - 100
- Escherichia coli ATCC 25922 (WDCM 00013): ≥ 1000
- Escherichia coli ATCC 8739 (WDCM 00012): ≥ 1000
- Proteus mirabilis ATCC 29906 (WDCM 00023): ≥ 1000
Growth on mCCDA
- Campylobacter coli ATCC 43478 (WDCM 00004):
>10 CFU
- Campylobacter jejuni ATCC 33291 (WDCM 00005): >10 CFU
- Campylobacter jejuni ATCC 29428 (WDCM 00156): >10 CFU
Growth on Tryptic soy agar
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Proteus mirabilis ATCC 29906 (WDCM 00023): no growth
Colony colour
- Campylobacter coli ATCC 43478 (WDCM 00004):
greyish
- Campylobacter jejuni ATCC 33291 (WDCM 00005): greyish
- Campylobacter jejuni ATCC 29428 (WDCM 00156): greyish
Incubation:
Bolton broth: 5 hours at 44°C, afterwards 44 hours at 41.5°C, Anaerocult C
Confirmation on mCCDA: at least 44 hours at 41.5°C, Anaerocult C
Confirmation on Tryptic soy agar: 24 hours at 35°C, aerobic