Bordet Gengou HiVeg Agar Base, 2.5KG

This medium is recommended for the detection and isolation of Bordetella pertussis and Bordetella parapertussis. The original medium is modified by replacing 50% human or rabbit blood with 15% sheep blood to make the medium more enriched for detection of B. pertussis by the virtue of its haemolytic reaction. Enrichment of the basal media with 25% human blood aids in the detection of Mycobacterium species from small sputum inocula and in Streptomycin sensitivity testing. Appearance of Powder: Light yellow colored, may have slightly greenish tinge, homogeneous, free flowing powder Gelling: Firm, comparable with 2.0% Agar gel of MV175 or 1.6% Agar gel of MV175A Color and Clarity: Basal medium yields, light yellow colored clear to slightly opalescent gel, with addition of 15% v/v sterile defibrinated blood, cherry red colored, opaque gel forms in petri plates Reaction of 4.0% w/v of MV175 or 3.6% w/v of MV175A aqueous solution is pH 6.7 ± 0.2 at 25°C Cultural characteristics observed after an incubation at 35 - 37°C for 3-4 days with added glycerol and 15% v/v sterile defibrinated blood