Brilliant Green HiVeg Agar, 5KG

Hiveg medium used for enumeration of coliform bacteria from materials of sanitary importance. The medium contains a combination of brilliant green and bile, which is highly selective for coliforms, inhibiting most of the gram-positive bacteria including lactose fermenting Clostridia and some gram-negative bacteria. Erioglaucine and basic fuchsin together form the indicator system of the medium. When the pH is neutral, color of the medium is blue while acid production from lactose turns the medium pink and colonies appear pink to deep red depending on the pH change. It is recommended that the medium be prepared just prior to use and if the medium has to be stored, it should be kept in dark. Application: For Isolation, Differentiation & Enumeration of Coliform Bacteria in Water & Wastewater Appearance of powder: Light purple colored, homogeneous, free flowing powder Gelling: Firm, comparable with 1.0% Agar gel Color and Clarity: Bluish purple colored, slightly opalescent gel forms in petri plates Reaction of 2.07% w/v aqueous solution is pH 6.9 ± 0.2 at 25°C Cultural characteristics observed after an incubation at 35-37°C, for 18-24 hours