Cell Line Origin
A rat pancreatic Beta cell: RINm5F hybrid cell line. Insulin-secreting cell line
Cell Line Description
BRIN-BD11 is a hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma). BRIN-BD11 has been shown to be tumourigenic when transplanted into a SCID mouse host. BRIN-BD11 has been mycoplasma eradicated at ECACC and the stocks available for supply have undergone a further 10 passages without detection of mycoplasma. The cell line has applications in the study of pancreatic beta cell function.
DNA Profile
Not specified
Culture Medium
RPMI-1640 + 2 mM Glutamine + 10% FCS / FBS
Subculture Routine
Split sub-confluent cultures (70-80%) 1:8 to 1:10 , i.e., seeding at 2-4 x 104 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Population doubling approx 20hrs. At confluence 105 cells/cm2 can be expected.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
- UPC:
- 51343102
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 10033003-1VL
