General description
A potent inhibitor of Ca2+-stimulated myosin S1 ATPase (IC50 = ~ 5 µM) and reversibly blocks the gliding motility. It also weakens myosin’s interaction with F-actin. Much less effective in suppressing contraction in rat myocardial or rabbit slow twitch muscle and has no effect on platelet myosin II.
An aryl sulphonamide that acts as a potent inhibitor of Ca2+-stimulated myosin S1 actin-stimulated ATPase activity (IC50 = ~5 µM). Also blocks actin-stimulated ATPase activity with similar potency (IC50 = 5 µM). Reversibly blocks gliding motility of skeletal muscle myosin (IC50<2 µM). Less effective in suppressing contraction in rat myocardial and rabbit slow twitch muscle compared to skeletal muscle. Does not inhibit platelet myosin II.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Ca2+-stimulated myosin S1 ATPase
Product does not compete with ATP.
Reversible: yes
Target IC50: ~5 µM against Ca2+-stimulated myosin S1 ATPase
Packaging
Packaged under inert gas
Warning
Toxicity: Standard Handling (A)
Reconstitution
Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes
Cheung, A., et al. 2002. Nat. Cell Biol.4, 83.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 41141504
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 203895-5MG