Superior Blocking Buffer in PBST (Phosphate-buffered saline, pH 7.5 with 0.05% Tween -20), 500mL

Superior ™ Blocking Buffer contains a proprietary antiginically non-determinant protein for blocking non-specific sites during ELISA, membrane blotting, immunohistochemistry and other applications. Superior ™ Blocking Buffer is ideal for a high signal to background ratio in most system. Superior ™ Blocking Buffer uses a non-serum protein and does not contain biotin or other animal source proteins to interfere with immuno-complexes. Superior™ Blocking Buffer is suitable for assays that use avidin/streptavidin systems. Superior ™ Blocking Buffer for Precipitating Substrate is a modification of Superior ™ Blocking Buffer. This blotting buffer has been optimized for used in blotting protocols that use precipitating substrates, such as TMB (3, 3', 5, 5'-Tetramethylbenzidine) and BCIP (5-Bromo-4-Chloro-3'-Indolyphosphate p-Toluidine Salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrates. Superior ™ Blocking Buffer for Precipitating Substrate is not suitable for ELISA or immunohistochemistry staining. Available in multiple formats using TBS, PBS, TBS with 0.05% Tween ® -20 or PBS with .05% Tween ® -20. Also supplied as a convenient dry form that is stable at room temperature. Each dry format pack is makes 200ml Superior ™ Blocking Buffer. Features Non serum protein blocking agent Rapid blocking times; ~2 minutes for ELISA Multiple formats; ready-to-use liquid (500ml) or dry buffer packs (for 5 x 200ml) Available with and without detergent (Tween ® -20) Applications For blocking Western blot membranes (PVDF and nitrocellulose) For blocking and storage of ELISA plates For blocking prior to immunohistochemistry staining Superior ™ Blocking Buffer for Precipitating Substrate is not suitable for ELISA or immunohistochemistry staining.