BUFFER, WASH, 70ML

Corning® 3D Clear Tissue Clearing Reagent The improved in vivo relevancy of 3D cell culture has driven the adoption of these models in drug discovery. However, the imaging techniques used to characterize these models are highly limited. Due to the thickness and opacity of these 3D cellular structures, current imaging technologies cannot penetrate to the center of the tissues, resulting in only the outer 2 to 3 layers of cells being detected. This causes the dark centers often seen in images of 3D cell culture models, which proves highly problematic for accurate analysis as these outer cells are most exposed to compounds, nutrients and oxygen, and thus do not reflect the entire cell population. Corning 3D Clear tissue clearing reagent can be used as a tissue clearing technique designed specifically to support imaging for 3D cell culture models and plate-based high throughput processing. When paired with fluorescent labeling (e.g., fluorescent protein, immunofluorescence, chemical dyes) and high content confocal microscopy, Corning 3D Clear reagent allows for complete 3D cell culture model characterization and more accurate drug screening. Why use Corning 3D Clear Tissue Clearing Reagent? While there are dozens of tissue clearing techniques described in literature, there are few that have been demonstrated for use with 3D cell culture models. Additionally, most of these tissue clearing techniques are not designed for compatibility with microplates (e.g., iDISCO, BABB, uDISCO, 3DISCO) or high throughput processing (e.g., CLARITY), and many are not compatible with immunofluorescent labeling (ScaleS4, CUBIC) or have high toxicity (e.g., ClearT). Therefore, the Corning 3D Clear tissue clearing technique was designed to be a rapid, easy-to-use process that is compatible with microplates imaging, fluorescent protein, and immunofluorescence. To get started with Corning 3D Clear tissue clearing reagent, you can purchase the Corning 3D Clear starter kit or you can purchase the Corning 3D Clear reagent, and then make the required buffer reagents yourself. Benefits of Corning 3D Clear Tissue Clearing Reagent Rapid tissue clearing Easy-to-use No special equipment required Compatible with immunofluorescent (IF), fluorescent protein (FP), and other fluorescent labels Microplate and automation compatible Reversible for follow up 2D H&E/IHC