Butyl-s Sepharose(R) 6 Fast Flow

General description

Butyl-S Sepharose^™ 6 Fast FLow is the least hydrophobic medium in the Sepharose^™ Fast FLow hydrophobic interaction chromatography (HIC) range and is particularly useful during the initial stages of a purification process to remove the bulk of impurities.

Application

Butyl-S Sepharose^™ 6 Fast FLow is part of the Sepharose^™ Fast FLow HIC platform, which has been an industrial standard for HIC chromatography during recent decades. Butyl-S Sepharose^™ 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with butyl groups via a thio-ether linkage. It will exhibit a different selectivity compared to Butyl Sepharose^™ 4 Fast FLow and other HIC Sepharose^™ Fast FLow products.

This resin is designed for the binding and elution of relatively strong hydrophobic molecules at comparatively Low salt concentrations. Butyl?S Sepharose^™ 6 Fast FLow is particularly useful during the initial stages of a separation process to remove the bulk of impurities without stringent requirements for conditioning of the sample (e.g. adjustment of pH, salt concentration etc.)

Butyl-S Sepharose^™ 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Butyl-S Sepharose^™ 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

• Binds and elutes relatively strong hydrophobic molecules at comparatively Low salt concentrations
• The least hydrophobic medium in the well proven Sepharose^™ Fast FLow HIC range
• Purifies recombinant Hepatitis B virus surface antigen from CHO cells
• Developed and optimized in cooperation with a process-scale manufacturer of biopharmaceuticals
• The hydrophilic nature of the base matrix ensures Low levels of non-specific binding.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva