C. botulinum Isolation HiVeg Agar Base, 500G

C. botulinum Isolation HiVeg™ Agar Base is recommended for rapid, selective isolation of Clostridium botulinum from food samples. This medium is formulated as per the recommendation of APHA. Botulinal toxin is heat-labile. Therefore the test samples and cultures should be maintained at refrigeration temperatures. The pH of the toxic material should also be maintained at a slightly acidic pH since botulinal toxin is less stable at alkaline pH. Egg yolk emulsion added in the medium helps in detecting lecithinase, lipase and proteolytic activity. Lecithinase degrades lecithin present in the egg yolk producing an insoluble, opaque precipitate in the medium surrounding the growth . Lipase break down free fats present in the egg yolk causing an iridescent (oil on water) sheen to form on the surface of the colonies. This medium is prepared by replacing animal peptones with vegetable defined peptones to avoid BSE/TSE/GMO risk. Appearance of powder: Light yellow colored, may have slightly greenish tinge, homogeneous, free flowing powder Gelling: Firm, comparable with 2.0% Agar gel Color and Clarity: Basal medium yields yellow colored slightly opalescent gel. With addition of egg yolk emulsion (FD045) and CBI supplement (FD049),light yellow colored opalescent gel forms in petri plates Reaction of medium (7.4 gm in 90 ml distilled water) is pH 7.4 ± 0.2 at 25°C Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours under anaerobic conditions with added Egg Yolk Emulsion (FD045) and CBI Supplement (FD049)