C-Chip Hemacytometer, Burker Turk (C08-0606-297)

Applications: Cell counts in cerebrospinal fluid (CSF), rare cells Blood analysis (Hematology): Blood cell counting Cell culture: Cell concentration measurement, Cell viability test Microbiology: Fungal spores counting Calculation The full grid on a hemocytometer contains 9 squares, each of which is 1 micro meter (mm) square. Large cells are counted using the four large corner squares (and the middle one). For a dense suspension of small calls, the four 1/25 sq. mm corners plus the middle square and the central square are used. It consists of 9 large squares, each measuring 1 x 1 mm, with the depth of chamber of 0.1 mm. Each square has a total volume of 0.1 mm 3 or 10-4cm 3 . The central square is divided into 25 small squares with triple lines and four corner squares divided into 16 small squares. Each large square has a surface area of 1.0mm 2 , and a chamber depth of 0.1mm. There are 1000 mm 3 per ml, each large square represents a volume of 0.0001ml, that equates to 1/ 0.0001ml = 10,000 = (10 4 ). The volume factor is 10,000.