General description
Recombinant, human MET (amino acids 956-1390) (Genebank entry NM_000245) fused at the N-terminus to a GST-His6-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. c-Met is a receptor tyrosine kinase activated by hepatocyte growth factor (HGF)/scatter factor (SF). MET is a plasminogen-like protein thought to be a humoral mediator of liver regeneration.
Recombinant, human c-MET (amino acids 956-1390) (Genebank entry NM_000245) fused at the N-terminus to a GST-His6-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. c-Met is a receptor tyrosine kinase activated by hepatocyte growth factor (HGF)/scatter factor (SF). c-MET is a plasminogen-like protein thought to be a humoral mediator of liver regeneration.
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to Poly(Ala, Glu, Lys, Tyr)6:2:5:1 per min at 30°C, using variable concentrations of ATP (0.1-1.48 µM).
Physical form
In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol, pH 8.0.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Palka, H.L., et al. 2003. J. Biol. Chem.278, 5278.
Vadnais, J., et al. 2002. J. Biol. Chem.277, 48342.
Crostella, L., et al. 2001. Oncogene20, 3735.
Guiton, M., et al. 1994. J. Biol. Chem269, 30370.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
recombinant: expressed in baculovirus infected Sf21 cells. Quality Level: 100. form: liquid. specific activity: ≥. 30 . units/mg protein. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. shipped in: wet ice. storage temp.: −. 70°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.- UPC:
- 41106608
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 325885-10UG