C106

Cell Line Origin

Human colorectal adenocarcinoma, moderately well differentiated, Dukes′ stage A

Cell Line Description

The C106 cell line was established from a 78-year old female patient with a moderately well differentiated adenocarcinoma of the rectum classified as Dukes′ stage A.

DNA Profile

STR-PCR Data:
Amelogenin: X
CSF1PO: 10,11
D13S317: 8,14
D16S539: 12,13
D5S818: 12
D7S820: 8,10
THO1: 6
TPOX: 10,11
vWA: 18

Culture Medium

Iscove′s Modified Dulbecco′s Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C. C106 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.