Cell Line Origin
Mouse RIII mammary tumour
Cell Line Description
Non-transformed clone from a RIII mouse. Supernatant fluid is negative for RT activity. Useful for visualisation of sarcoma virus-induced foci, in vitro quantitative assay for bovine papilloma virus. Suitable host for transformation with bovine papilloma virus DNA plasmids.
Application
Virus and transformation studies: Sarcoma virus, Bovine papilloma virus
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:5 to 1:15 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: mouse breast. growth mode: Adherent. karyotype: Not specified. morphology: Epithelial. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 51201649
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 90060504-1VL
- Temperature Control Device:
- Yes
