Cell Line Origin
Human Caucasian skin, Cri du Chat syndrome
Cell Line Description
Derived from a biopsy of an 11 year old Caucasian male with Cri du Chat syndrome. Cells have a limited lifespan of approximately 35 passages. Autoradiographic studies demonstrated that a distinction could be made between chromosome 4 and 5 in the Cri du chat syndrome by alternative DNA replication pattern and length of long and short arms.
Application
Genetic studies
Culture Medium
McCoy′s 5a + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Other Notes
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biological source: human skin. growth mode: Adherent. karyotype: 2n : 46. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51241232
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 90112604-1VL
