Cell Line Origin
Mouse C3H muscle myoblast
Cell Line Description
Subclone from myoblast line established from normal adult C3H mouse leg muscle. Differentiates rapidly; produces extensive contracting myotubes expressing characteristic muscle proteins. Provides model to study in vitro myogenesis and cell differentiation.
Application
C2C12 Cell Line has been used to study the the expression of OXT and OXTR (oxytocin and its receptor) genes and proteins and in differentiation studies.
Culture Medium
DMEM + 2mM Glutamine + 10-15% Foetal Bovine Serum FBS / FCS.
Subculture Routine
Split semi-confluent cultures 1:3 to 1:6 i.e. seeding at 1-2x1,000 cells/cm2 using 0.25% Trypsin or Trypsin/EDTA; 5% CO2; 37°C. Do not allow cells to reach confluence.
Other Notes
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biological source: mouse muscle. growth mode: Adherent. karyotype: Not specified. morphology: Myoblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 12352206
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 91031101-1VL
