Cell Line Origin
Mouse C3H embryo
Cell Line Description
Derived by exposing C3H/10T1/2 clone 8 cells with 3-methylcholanthrene for 24 hours. Subsequently they lost the property of contact inhibition. Inoculation of 100,000 cells or more into syngenic mice has been reported to produce fibrosarcoma.
Application
Tumourigenicity studies.
Culture Medium
BME (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: mouse embryo. growth mode: Adherent. karyotype: Not specified. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 11131607
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 90110523-1VL