C527 (C09-1120-943)

C527 is a is a pan DUB enzyme inhibitor, with a high potency for the USP1/UAF1 complex ( IC 50 =0.88 μM).In VitroPretreatment of USP1/UAF1 with C527 resulted in inhibition of its enzyme activity with an IC 50 of 0.88±0.03 μM. C527 inhibits the DUB activity of the USP12/USP46 complex and other DUB enzymes in vitro . However, the IC 50 of C527 for these DUB enzymes was higher in comparison with USP1/UAF1 complex. C527 has considerably less inhibitory effect on UCH-L1 and UCH-L3, a different subclass of DUB enzymes. C527 treatments causes an increase in the levels of Ub-FANCD2 and Ub-FANCI. Pretreatment of cells with the C527 causes an enhancement in the cytoxicity of mitomycin C and camptothecin. C527 treatments lead to an increase in ubiquitinated forms of FANCD2 and FANCI, cause a decrease in homologous recombination activity, and sensitize cells to DNA damaging agents. MCE has not independently confirmed the accuracy of these methods. They are for reference only.Form:SolidIC50& Target:0.88 μM (USP1). Specifications and Purity: 99%. Molecular Formula: C17H8FNO3. Molecular Weight: 293.25. EC Number: 808-533-8. PubChem CID: 2307331. Isomeric SMILES: C1=CC=C2C(=C1)C(=O)C3=C(C2=O)OC(=N3)C4=CC=C(C=C4)F.