CALPAIN SUBSTRATE IV CELL-PERMEABLE FLUOROGENIC - CALBIOCHEM

General description

The non-permeant fluorogenic Calpain Substrate III (Cat. No. 208771) is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage. In addition to the expected improvement in permeability, the modified peptide also exhibits enhanced Calpain B sensitivity (k_cat = 0.2 s^-1, k_cat/K_M = 5000 M^-1 s^-1) when compared to the parental peptide (k_cat = 0.17 s^-1, k_cat/K_M = 680 M^-1 s^-1). Its suitability in detecting cellular Ca²⁺-dependent calpain activity is demonstrated both in intact Drosophila melanogaster S2 cells following ionomycin stimulation and in S2 lysates via direct Ca²⁺ addition.

The non-permeant fluorogenic Calpain Substrate III (Cat. No. 208771) is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage. In addition to the expected improvement in permeability, the modified peptide also exhibits enhanced Calpain B sensitivity (k_cat = 0.2 s^-1, k_cat/K_M = 5000 M^-1 s^-1) when compared to the parental peptide (k_cat = 0.17 s^-1, k_cat/K_M = 680 M^-1 s^-1). Its suitability in detecting cellular Ca²⁺-dependent calpain activity is demonstrated both in intact Drosophila melanogaster S2 cells following ionomycin stimulation and in S2 lysates via direct Ca²⁺ addition.

The non-permeant fluorogenic Calpain Substrate III is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

(DABCYL)-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-Glu(EDANS)-Arg-Arg-Arg-Arg-Arg-Arg-Arg-NH₂

Physical form

Supplied as a trifluoroacetate salt

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Banoczi, Z., et al. 2008. Bioconjug. Chem.19, 1375.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany