cAMP Biotrak(TM) EIA, (Non-Acetylation Protocol)

General description

EIAs and ELISAs are convenient, high-sensitivity, peroxidase-based technologies that enable the amount of protein present in a sample to be measured in a single day. Novel lysis reagents have been developed (Lysis Reagents 1 and 2) to facilitate simple and rapid extraction of cellular molecules.

The Biotrak^™ cAMP competitive enzymeimmunoassay system is specifically designed for research purposes. The kit includes protocols using novel lysis reagents in order to facilitate simple and rapid extraction of cAMP from cell cultures. These components avoid the requirement for traditional, time-consuming extraction procedures and obviate the need for removal of extraction reagents prior to measurement. It combines the use of a peroxidase-labeled cAMP conjugate, a specific antiserum which can be immobilized on to precoated microplates, and a one-pot stabilized substrate solution.

Lysis reagent 1 hydrolyzes cell membranes to release intra-cellular
cAMP. Lysis Reagent 2 sequesters the key component in Lysis
Reagent 1 and ensures cAMP is free for subsequent analysis. The
detergent/sequestrant complex does not interfere with antigen:
antibody binding. Lysis Reagent 1 is simply added to cultured cells,
followed by a 5 to 10 min incubation before assay. The
antiserum is reconstituted with Lysis Reagent 2. The assay is based
on competition between unlabeled cAMP and a fixed quantity of
peroxidase-labeled cAMP, for a limited number of binding sites on a
cAMP specific antibody.

Application

cAMP Biotrak^™ EIA, (Non-Acetylation Protocol) has been used to measure intracellular cyclic adenosine monophosphate (cAMP) levels.

Features and Benefits

• Elimination of inconvenient, time-consuming extraction
  procedures.
• Flexible method-choice of assay protocols.
• Range 25 - 6400 fmol/well (non-acetylation protocol).
• Rapid assay protocol.
• Nonradioactive.
• Specific for cAMP.
• Precise and accurate measurement
• Ready-to-use substrate.
• Colour coded reagents

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Biotrak is a trademark of Cytiva