General description
Cap 1 solution consists of n-methylimidazole, lutidine, and acetonitrile (ACN).
Application
Cap 1 is suitable for use after the phosphoramidite reaction/coupling step.
Biochem/physiol Actions
Cap 1 solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.
Preparation Note
Acetonitrile / Lutidine / N-Methylimidazole 8/1/1 (v/v/v)
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- UPC:
- 41116134
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- Yes
- MPN:
- L040150-2.5L
