General description
Cap A solution contains acetic anhydride and tetrahydrofuran (THF). It is used after the phosphoramidite reaction/coupling step.
Biochem/physiol Actions
Cap A solution aborts chains bearing unreacted 5′ hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′ hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.
Preparation Note
Acetic Anhydride / Tetrahydrofurane 9.1/90.9 (v/v)
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- UPC:
- 41116134
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- Yes
- MPN:
- L040080-4L
