Cell Line Origin
BALB/c mouse spleen x Mouse P3X63Ag8.653
Cell Line Description
CAR-2/3F4, also known as 2/3 F4, was formed by fusing P3X63Ag8-653 mouse myeloma cells with spleen cells from a BALB/c mouse immunised six times with 20μg human thioredoxin (TRX) at monthly intervals. Human TRX was cross-linked to Keyhole Limpet Haemocyanin for this purpose. The antibody is directed against monomeric and polymeric forms of human TRX and shows no cross-reactivity to bacterial TRX or human glutaredoxin. CAR-2/3F4 has been applied as capture antibody in a chemiluminescent detection assay for free human TRX. CAR-XP10 (Sigma Catalogue number. 97011601) can be used as second antibody in this assay.
Immunogen
Human Thioredoxin cross linked to Keyhole Limpet Haemocyanin
Application
Immunohistochemistry, Western blots
Culture Medium
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 15% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 2-9 x 100,000 cells / ml; 5% CO2; 37°C; start culture on mouse macrophage feeder layer
Other Notes
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biological source: Mouse x Mouse Hybridoma. ImmunoDon: BALB/c mouse spleen. Myeloma: P3X63Ag8.653. Reactivity: Anti-Thioredoxin, human. technique(s): cell culture | mammalian: suitable. isotype: IgG1. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41116127
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 97011602-1VL