Cell Line Origin
BALB/c mouse spleen x Mouse P3X63Ag8.653
Cell Line Description
The hybridoma cell line CAR-XP10, also known as XP10, was produced by fusing P3X63Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse immunised six times with 20μg human thioredoxin (TRX) at monthly intervals. Human thioredoxin was cross-linked to Keyhole Limpet Haemocyanin for this purpose. The secreted antibodies react with monomeric and polymeric forms of human TRX and show no cross-reactivity to bacterial TRX or human glutaredoxin. CAR-XP10 has been used as second antibody in chemiluminescent detection assays for full human TRX. CAR-2/3F4 (Sigma Catalogue number. 97011602) can be applied as capture antibody.
Immunogen
Human Thioredoxin cross-linked to Keyhole Limpet Haemocyanin
Application
Immunohistochemistry, Western blots
Culture Medium
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 15% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells / ml; 5% CO2; 37°C; start culture on mouse macrophage feeder layer
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
biological source: Mouse x Mouse Hybridoma. ImmunoDon: BALB/c mouse spleen. Myeloma: P3X63Ag8.653. Reactivity: Anti-Thioredoxin, human (monomeric and polymeric forms). technique(s): cell culture | mammalian: suitable. isotype: IgG2a. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41116127
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 97011601-1VL
