Carrier RNA (C007B-555123)

Aladdin's Carrier RNA is mainly used for improving RNA recovery rate in RNA extraction from microsamples, especially from viral samples.his product is compatible with most viral RNA extraction kits, such as Aladdin's RNAeasy™ Viral RNA Isolation Kit with Spin Column . It not only improves the binding and elution efficiency of viral RNA in RNA purification process, but also prevents RNA from degradation by residual RNase. Carrier RNA has better effect in improving RNA recovery rate when the viral RNA content is relatively low. In addition, the commonly used EP tube is polypropylene, and there is electrostatic adsorption of RNA to the surface of the tube. The addition of Carrier RNA to the lysis buffer for RNA extraction can effectively eliminate the electrostatic adsorption and significantly improve the elution efficiency of RNA.Carrier RNA is not originated from virus and will not interfere with the subsequent detection of viral RNA.The concentration of Carrier RNA in this product is 1µg/µl.Precautions：Avoid repeated freeze-thaws. When using it for the first time, please make aliquots and store at -20℃ or -80℃.When handling this product, use RNase-free tips and EP tubes and wear disposable masks to avoid contamination by RNase.Carrier RNA is recommended for extraction of small-amount RNA or viral RNA. It is optional if the amount of RNA is large.Quantification of extracted RNA by the traditional methods is inaccurate when Carrier RNA is included. We recommend using qRT-PCR method for RNA quantification if necessary.This kit is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use：1. Each viral sample requires 2-5µg of Carrier RNA. It can be added directly to the lysis buffer provided in the RNA isolation kit. For example, for one viral sample, add 3µl of Carrier RNA (1µg/µl) to 300µl lysis buffer, mix well and then add to the viral sample.2. Proceed with RNA extraction as described in the manual of the RNA isolation kit.