General description
Casein is a phosphoprotein found in milk. It is classified into four subtypes, including αS1-, αS2-, β-, and κ-caseins.
Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes.
Application
Casein Blocking Buffer 10x has been used in:
- western blotting
- enzyme linked immunosorbent assay (ELISA)
- immunohistochemistry
Features and Benefits
- Better blocking performance than dried milk or BSA
- Easy-to-use concentrate
- Compatible with neutral and positively-charged membranes
- Compatible with chemiluminescent and colorimetric detection assays
Preparation Note
Dilute 10x Blocking Buffer with molecular biology grade water before use.
- UPC:
- 51263311
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- B6429-500ML
