General description

Casein hydrolysate is commonly used in microbiology as a nitrogen source and a supplement in microbial culture media. It is produced by the digestion of casein with hydrochloric acid and contains a complex mixture of peptides and amino acids. Casein hydrolysate is particularly useful in media formulations for the growth of fastidious bacteria that require specific nutrients or additional growth factors. It provides a rich source of nitrogen, which is essential for microbial growth and protein synthesis. Casein hydrolysate can also be used as a substitute for complex peptones in certain applications, as it provides similar nutritional content.

Application

Casein hydrolysate has various applications in sterility testing media or biochemical characterization as it is utilized by a wide variety of microorganisms. It can also be used in the production of certain antibiotics, toxins, bacteriocins, and enzymes. It is especially suited for the large-scale cultivation of Diphteria bacteria, Tetanus bacilli and Streptococci. In industrial fermentations it favors a high yield of biomass, particularly for certain yeasts and fastidious bacilli.

Analysis Note

Appearance: Lightly yellow to lightly brownishyellow fine powder.
Identity (NIR): passes test
Total nitrogen (N) (Kjeldahl): 7.0 - 8.5 %
pH-value (5 %; water): 4.7 - 7.0
Amino nitrogen (as N): 5.0 - 6.5 %
Sulfated ash (800 °C): ≤ 58 %
Loss on Drying (105°C): ≤ 6.0 %
Suitability for microbiology: passes test