General description
Recombinant, human caspase-2 expressed in E. coli as a single polypeptide chain. Subsequently undergoes spontaneous auto-processing to yield the native active enzyme. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. Cleavage sites are located at Asp316, Asp330, and possibly Asp152. MW: 12000 and 19000.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will cleave 1.0 nmol of Ac-VDVAD-pNA (Cat. No. 218820) per h at 37°C, pH 7.2.
Reconstitution
Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.
Other Notes
Talanian, R.V., et al. 1997. J. Biol. Chem.272, 9677.
Wang, L., et al. 1994. Cell78, 739.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 41106501
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 218813-100U