General description
Fluorogenic CPP32/Apopain substrate. Cleavage of this substrate by CPP32/Apopain shows Michaelis-Menten kinetics (Km = 9.7 ± 1.0 µM). Cleavage can be monitored by excitation at 380 nm and emission at 460 nm.
Fluorogenic caspase-3 substrate. Cleavage of this substrate by caspase-3 shows Michaelis-Menten kinetics (Km = 9.7 ± 1.0 µM). Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10.
Biochem/physiol Actions
Cell permeable: no
Km = 9.7 ± 1.0 µM for cleavage of this substrate by caspase-3
Primary Target
Fluorogenic caspase-3 substrate
Product does not compete with ATP.
Reversible: no
Warning
Toxicity: Standard Handling (A)
Sequence
Ac-Asp-Glu-Val-Asp-AMC
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Hermission, M., et al. 2000. Oncogene 19, 2338.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Nicholson, D.W., et al. 1995. Nature 376, 37.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 41116113
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 235425-1MG
- CAS:
- 169332-61-0
