Cathepsin B Inhibitor Screening Kit

DescriptionCathepsin B (CTSB; EC 3.4.22.1) is a lysosomal cysteine proteinase involved in intracellular degradation and turnover of proteins. It is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP is a causative factor in Alzheimer′s disease. CTSB has also been implicated in the invasion and metastasis of certain cancers.The Cathepsin B Inhibitor Screening Kit is a simple assay for screening potential inhibitors of CTSB. CTSB activity is measured by cleaving a synthetic 7-amino-4-trifluoromethylcoumarin (AFC)-based substrate to yield AFC, a fluorescent product (λex= 400/λem= 505 nm), proportional to the enzymatic activity present.Suitability: Suitable for the screening of inhibitors of Cathepsin B.Principle: The Cathepsin B Inhibitor Screening Kit is a simple assay for screening potential inhibitors of CTSB. CTSB activity is measured by cleaving a synthetic 7-amino-4-trifluoromethylcoumarin (AFC)-based substrate to yield AFC, a fluorescent product (λex= 400/λem= 505 nm), proportional to the enzymatic activity present.}Preparation instructionsPrincipleThe Cathepsin B Inhibitor Screening Kit is a simple assay for screening potential inhibitors of CTSB. CTSB activity is measured by cleaving a synthetic 7-amino-4-trifluoromethylcoumarin (AFC)-based substrate to yield AFC, a fluorescent product (λ/. Specifications and Purity: Sufficient for 100 Fluorometric tests. Related Document: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/C486413-SCI_8d8875d29e02c20b396fd723ed2fff6b.pdf.