CATHEPSIN S ACTIVE HUMAN

General description

Human cathepsin S (GenBank Accession No. NM_004079.3), CD33 signal peptide(amino acids 1-16) + cathepsin S (amino acids 17-331), with C-terminal HIS tag, MW = 37 kDa, expressed in FreeStyle 293-F cells.

Application

Active human cathepsin S is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human cathepsin S has been used in a study to assess the pathogenesis of Alzheimer′s disease. Active human cathepsin S has also been used in a study to investigate the optimization of selectivity of Azepanone-based inhibitors.

Unit Definition

One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C

Physical form

Formulated in 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 3 mM DTT, and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Legal Information

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