General description
Recombinant, human procathepsin V (amino acids 18-334) fused at the N-terminus to a CD33 signal sequence (amino acids 1-16) and at the C-terminus to a His•Tag
Recombinant, human procathepsin V (amino acids 18-334) fused at the N-terminus to a CD33 signal sequence (amino acids 1-16) and at the C-terminus to a His•Tag
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will cleave 1 pmol the fluorescent substrate Z-LR-AMC per min at 25°C, pH 5.5.
Physical form
In 800 mM NaCl, 25 mM sodium acetate, 0.8 M NaCl, pH 5.0.
Reconstitution
To activate rhCathepsin V, pre-incubate it at 20 µg/ml in 25 mM NaOAc, 100 mM NaCl, 5 mM DTT, pH 5.5 at room temperature. Following initial thaw aliquot and freeze (-70°C).
Other Notes
Tolosa, E., et al. 2003. J. Clin. Invest.112, 517.
Turk, V., et al. 2001. EMBO J.20, 4629.
Somoza, J.R., et al. 2000. Biochemistry39, 12543.
Bromme, D., et al. 1999. Biochemistry38, 2377.
Adachi, W., et al. 1998. Invest. Ophthalmol. Vis. Sci.39, 1789.
Santamaria, I., et al. 1998. Cancer Res.58, 1624.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 12352206
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 219467-10UG