CC HPLC guard column (analytical), NUCLEOGENr, length: 30 mm

Silica-based HPLC phases for anion exchange chromatography of nucleic acids Features Base material silica, particle size 7 µm DEAE anion exchanger NUCLEOGEN® 60-7 DEAE: Pore size 60 Å Capacity 200 A260/mL (~ 300 A260 for a 125 x 4 mm ID column, 1875 A260 for a 125 x 10 mm ID column) For separation of oligonucleotides up to chain lengths of 40 bases with recoveries > 95 % Preparative separations possible when using higher flow rates and longer gradient times NUCLEOGEN® 500-7 DEAE: Pore size 500 Å Capacity 730 A260 for a 125 x 6 mm ID column, 1940 A260 for a 125 x 10 mm ID column For the separation of tRNA, 5S RNA, viroids and messenger RNA in the intermediate molecular weight range (25,000–1,000,000 daltons) with recoveries > 95 % NUCLEOGEN® 4000-7 DEAE: Pore size 4000 Å Capacity 120 A260 for a 125 x 6 mm ID column, 350 A260 for a 125 x 10 mm ID column For the separation of plasmids, DNA restriction fragments, ribosomal RNA, messenger RNA and viral RNA, i.e., very high molecular weight nucleic acids (e.g., 1–50 megadaltons) Eluent in column: methanol