Cell Line Origin
Human Caucasian lung
Cell Line Description
Established from normal lung tissue of a 35 year old Caucasian male who died of a class 1-2 astrocytoma. The donor who was maintained for organ transplantation prior to removal of the lung. The cells will undergo a further 11 doublings.
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,12
D16S539: 13
D5S818: 11,13
D7S820: 7,11
THO1: 9
TPOX: 10,11
vWA: 18,20
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: human lung. growth mode: Adherent. karyotype: 2n : 46. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51162720
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 86090301-1VL
