Cell Line Origin
Human Caucasian astrocytoma
Cell Line Description
Established from a Grade IV astrocytoma from a 68-year old Caucasian female. Cells exhibit strong perinuclear acid and phosphatase activity, glial fibrillary acidic protein present in 70-80% cells. HLA-DR present on approximately 20-30% cells after 48 hours culture.
Application
HLA profiles
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: human brain. growth mode: Adherent. karyotype: Not specified. morphology: Astrocytoma. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51202405
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 90021502-1VL