General description
Contents
25 mM solution (12.38 mg/ml), 100x concentration
Application
Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots:
- Southern blot
- Northern blot
- Dot blot
- Colony and plaque hybridization
- Gel shift assays
Features and Benefits
CDP-Star is a chemiluminescent substrate for alkaline phosphatase that enables fast, ultra-sensitive detection of biomolecules by producing extremely rapid visible light. Light emission is recorded on X-ray film or on luminescence imager systems.
- Save time with a fast, ultra-sensitive substrate.
- Easily strip and reprobe membranes.
- Take multiple exposures of up to two days for each experiment.
- Reuse the substrate by filtering and storing in sodium azide.
Quality
Purity: Purified by HPLC.
Preparation Note
Storage conditions (working solution): Washing Buffer: 15 to 25 °C, stable
Maleic Acid Buffer: 15 to 25 °C, stable
Detection Buffer: 15 to 25 °C, stable
Blocking Solution: always prepare freshly
Antibody Solution: 2 to 8 °C
Other Notes
- First exposure to X-ray film should be 5-15 minutes. Examine film and then determine other exposure times based on initial result.
- No preincubation step required.
- Apply substrate quickly to avoid gray shadows from substrate drops and drying of membrane (leads to uneven, high background).
- Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
- Exposure times are shorter than with CSPD.
- Do not use nitrocellulose membranes.
- Repeat exposures can be made up to two days after the addition of substrate.
For life science research only. Not for use in diagnostic procedures.
Legal Information
CDP-Star is a registered trademark of Tropix, Inc.
- UPC:
- 42202603
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 11685627001
