Fluorochromes

Cell Line Origin Rat hepatoma Hybrid [H5xFao] Cell Line Description HF1-5 is a subclone of HF1 selected for it&#8242s ability to grow in glucose free medium. The entire group of hepatic functions are re-expressed.

Kit for the detection and quantification of apoptosis at the single-cell level, based on labeling of DNA strand breaks (TUNEL technology) analysis by fluorescence microscopy or flow cytometry. Widely used methods to determine apoptosis include the

Methyl 2-amino-5-fluoronicotinate.

Methyl 2-amino-5-fluoronicotinate.

Methyl 2-amino-5-fluoronicotinate.

methyl 2-fluoro-6-methoxynicotinate.

Methyl 5-fluoro-2-methoxynicotinate.

Methyl 5-fluoro-2-methoxynicotinate.

A fluorescence energy transfer (FRET) peptide substrate with MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination. Useful for the detection of MMP activity (k cat /K m = 619,000 M -1 s -1 for MMP-2, 206,000 M -1 s -1 for

An excellent quenched fluorescence substrate for MMP-1 and MMP-9 (excitation maximum: 365 nm emission maximum: 450 nm). Both enzymes cleave this substrate between Gly and Cys(Me), liberating a fluorescent cleavage product.

A fluorescence resonance energy transfer (FRET) peptide substrate for the detection of MMP activity (k cat /k m = 730,000 M -1 s -1 for MMP-14 (MT1-MMP) and 36,000 M -1 s -1 for MMP-11 (ST3) pH = 7.5 at 25&#176C).

A quenched fluorescence substrate for MMP-2 (gelatinase A k cat /K m = 3.97 x 10 5 M -1 s -1 ).