CELL PROLIFERATION ELISA BRDU CHEM.LUM.

General description

Chemiluminescent immunoassay for the quantification of cell proliferation, based on the measurement of BrdU incorporation during DNA synthesis:  a nonradioactive alternative to the [³H]-thymidine incorporation assay.

Specificity

The antibody conjugate reacts with the thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) and with BrdU incorporated into DNA. For binding to BrdU incorporated into the DNA, the BrdU-labeled DNA has to be denatured. The antibody does not cross-react with any endogenous cellular components such as thymidine, uridine, or DNA.

Application

Cell Proliferation ELISA, BrdU (chemiluminescent) has been used:

• for bromodeoxyuridine (BrdU) proliferation assay in ReN and primary astrocytes
• to test the effect on imatinib on the proliferation of neuroblastoma cells using BrdU incorporation assay
• to check the effect of palbociclib on cell cycle inhibition using BrdU incorporation in pancreatic patient-derived primary cell lines

The Cell Proliferation ELISA, BrdU (chemiluminescent) belongs to the second, improved generation of kits for measuring DNA synthesis. It is a precise, fast, and simple nonradioactive alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell systems. For example:

• Detection and quantification of cell proliferation induced by growth factors and cytokines
• Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation in environmental and biomedical research, and in the food, cosmetic, and pharmaceutical industries
• Measurement of the immunoreactivity of lymphocytes, stimulated by mitogens or antigens
• Analysis of the chemosensitivity of tumor cells to different cytostatic drugs in medical research

Packaging

1 kit containing 7 components.

Components

1. BrdU Labeling Reagent, 1,000x conc
2. FixDenat
3. Anti-BrdU-POD 4. Antibody Dilution Solution
4. Washing Buffer PBS, 10x conc.
5. Substrate Component A
6. Substrate Component B

Other Notes

For life science research only. Not for use in diagnostic procedures.