CELL PROLIFERATION ELISA BRDU (COLORIM.)

General description

Colorimetric immunoassay for the quantification of cell proliferation, based on the measurement of BrdU incorporation during DNA synthesis:  a nonradioactive alternative to the [³H]-thymidine incorporation assay.

Specificity

The antibody conjugate reacts with the thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) and with BrdU incorporated into DNA. For binding to BrdU incorporated into the DNA, the BrdU-labeled DNA has to be denatured. The antibody does not cross-react with any endogenous cellular components such as thymidine, uridine, or DNA.

Application

For research use only. Not for use in diagnostic procedures.
The Cell Proliferation ELISA, BrdU (colorimetric) belongs to the second, improved generation of kits for measuring DNA synthesis. It is a precise, fast, and simple colorimetric alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell systems. For example:

• Detection and quantification of cell proliferation induced by growth factors and cytokines
• Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation in environmental and biomedical research, and in the food, cosmetic, and pharmaceutical industries
• Measurement of the immunoreactivity of lymphocytes, stimulated by mitogens or antigens
• Analysis of the chemosensitivity of tumor cells to different cytostatic drugs in medical research
• Testing of biocompatibility of various scaffolds, employed in bone tissue engineering, for bone cell growth

Packaging

1 kit containing 6 components.

Preparation Note

Working concentration: The kit antibody (Anti-BrdU-peroxidase) has, after reconstitution, a concentration of 7.5 U/ml, after dilution 0.075 U/ml. Instead of the kit antibody, you can also use the Anti-BrdU-Antibody, Fab fragments.This antibody is double-concentrated, so you have to dilute it after reconstitution in 1 ml with 1:200.
Working solution: BrdU labeling solution

Dilute BrdU labeling reagent 1:100 with sterile culture medium (resulting concentration: 100 μM BrdU).
For one 96-well MP, 1 ml BrdU labeling solution is required if the cells were cultured in 100 μl /well (10 μl/well) and 2 ml BrdU labeling solution is required if the cells were cultured in 200 μl/well (20 μl/well).

Anti-BrdU-peroxidase stock solution

Dissolve Anti-BrdU-peroxidase in 1.1 ml double-dist. water for 10 minutes and mix thoroughly.

Anti-BrdU-peroxidase working solution

Dilute Anti-BrdU-peroxidase stock solution 1:100 with antibody dilution solution. For one 96-well MP dilute 100 μl Anti-BrdU-peroxidase stock solution in 10 ml antibody dilution solution

Washing solution

Dilute Washing buffer concentrate 1:10 with double-dist. water.
For one 96-well MP dilute 10 ml Washing buffer concentrate with 90 ml double-dist. water.
Storage conditions (working solution): BrdU labeling solution
The undiluted BrdU labeling reagent (1000x): At 2 to 8 °C for several months protected from light.
The diluted BrdU labeling reagent: At 2 to 8 °C stable for several weeks. Store protected from light. For long-term storage it is recommended to store the BrdU labeling solution in aliquots at -15 to -25 °C.
Anti-BrdU-peroxidase stock solution
At 2 to 8 °C for several months. For long-term storage it is recommended to store the solution in aliquots at -15 to -25 °C.
Anti-BrdU-peroxidase working solution
Prepare shortly before use. Do not store.
Washing solution
At 2 to 8 °C for several weeks.

Reconstitution

The working solution for the antibody should be phosphate buffered saline containing 1% BSA, pH 7.4

Other Notes

For life science research only. Not for use in diagnostic procedures.