CellASIC ONIX2 Microfluidic System

General description

CellASIC^® ONIX2 Microfluidic System is an automated platform for precise manipulation of multiple key cell culture parameters, enabling measurement of cellular responses to pre-programmed media, temperature, and gas environment changes. It provides detailed information about live cell processes with ultra-high quality images and complete environment control. The system utilizes high quality, optically clear microfluidic plates and intuitive software, while integrating with a broad range of inverted microscopes to allow continuous, high magnification observation of live cells—as they react to their environment in time.

Includes:

• Controller System: Small footprint, integrated microincubator controller maintains fluid movement, reagent additions, temperature, and gas conditions.
• Microfluidic Plates: Application specific plates bring new cell culture capabilities for live cell imaging.
• Manifold: Low-profile, high quality, high optical clarity culture chambers and manifold, mount easily on an inverted microscope—without the need for a bulky environmental chamber.
• Software: Intuitive software enables quick and easy set-up of detailed protocols for truly automated hands-free cell culture.

• Automatically control flow rates, gas and temperature shifts, standing gradients, nutrient/drug additions, and media changes.
• Eight critical cell culture parameters can be controlled by the system.
• Temperature, oxygen level, growth factors, humidity, pH, osmolality, glucose, ECM and adhesion.

• High resolution imaging through optical glass bottom and chamber structures.
• Ability to run multiple independent experiments simultaneously.
• Continuous access to nutrients and ongoing removal of waste, promoting optimal cell health.

• Manifold seals to the microfluidic plate via “one-touch” vacuum mechanism.
• Pressure-driven flow of liquids to the cell chamber provides high precision even at very small volumes.
• No contact between the flow system and the biological solutions (on the plate) prevents contamination.
• Low-profile manifold is easy to adapt to any inverted microscope stage.

• Clearly defined protocols with all experimental parameters saved in one file.
• Convenience and reproducibility.
• Consistency and reliability.
• Error checking, self test, and data logging all make for better troubleshooting and serviceability.

Application

• Yeast single cell response.
• Bacterial single cell response.
• Bacterial biofilm dynamics.
• Cell response to hypoxic environments.
• Cell response to changing media conditions, drugs, and other stimulants.
• Host-pathogen interactions.
• Chemotaxis/migration in response to chemogradient.
• Setting optimal culture parameters for difficult cell types or long term experiments.
• Microscopy of 3D cell culture.

Legal Information

CELLASIC is a registered trademark of Merck KGaA, Darmstadt, Germany