Cetrimide Broth Base is recommended for cultivation of Pseudomonas aeruginosa from water samples using membrane filter technique. Pack size is 2.5KG

Cetrimide Broth Base is the modification of the formula designed by King, Ward and Raney (1) and is useful for the cultivation of P.aeruginosa . This medium is also selective as it contains cetrimide (Cetyl trimethyl ammonium bromide), which inhibits other bacteria except P.aeruginosa . ISO Committee recommends Cetrimide Broth Base (M862A) for cultivation of P. aeruginosa using membrane filter technique (2). Peptic digest of animal tissue provide necessary nutrients for P. aeruginosa . Cetyl trimethyl ammonium bromide (Cetrimide) is a quaternary ammonium compound, which inhibits bacteria other than Pseudomonas aeruginosa . The production of pyocyanin, a blue water-soluble pigment is stimulated by magnesium chloride and potassium sulphate (3, 4). Directions: Suspend 31.9 grams in 1000 ml distilled water. Heat, if necessary, to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 25 ml filter sterilized ethanol. Mix well before dispensing.