General description
CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.
Application
- 10mM CHAPS detergent has been used in the process of ion-exchange chromatography for improved purification.
- 0.5 w/v of CHAPS is a component of high salt buffer in protein purification alongside HEPES, NaCl and EDTA.
- HEPES buffer has been used as lysis buffer in immunoprecipitation, supplemented with 1% CHAPS.
- UPC:
- 41115720
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- C5070-1G
- CAS:
- 331717-45-4
