Charcoal Blood Agar Base is recommended for the cultivation of Bordetella pertussis for vaccine production and also for the maintenance of stock cultures. Addition of sterile defibrinated horse blood provides enrichment to the media. Penicillin inhbits contaminating bacteria. Cephalexin inhibits gram-positive organisms. Bordetella bronchiseptica, Bordetella parapertussis and Bordetella pertussis grows luxuriantlly on this medium while Staphylococcus aureus and Klebsiella pneumoniae are inhibited. Application: Used For The Cultivation of Bordetella Pertussis For Vaccine Production & Also For The Maintenance of Stock Cultures Appearance: Grey to greyish black homogeneous free flowing powder Gelling: Firm, comparable with 1.2% Agar gel Color and Clarity of prepared medium: Black colored, opaque gel with undissolved black particles forms in Petri plates Reaction of 5.45% w/v aqueous solution at 25°C. pH : 7.5±0.2 Cultural characteristics observed w/added sterile defibrinated blood and 100u/ml penicillin solution and 0.1% solution of 4:4 Diamido-diphenylamine hydrochloride, after an incubation at 35-37°C for 24-48 hours
- UPC:
- 51201647
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- M646-500G
