General description
Consanguineous: No
Homozygous: No
Cell Line Description
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
DNA Profile
HLA DRB1: 1001 11
HLA DRB3: 202
HLA DQAP: 1.3.4.1
HLA DQA1: 0501 0104
HLA DQB1: 0501 0301
HLA DPB1: 02012 3201
Serological Profile
HLA A: 11 19
HLA B: 7 40
Culture Medium
RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Subculture Routine
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
Other Notes
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biological source: human blood. Quality Level: 100. form: liquid. ethnicity: Thai. growth mode: Suspension. karyotype: Not specified. morphology: Lymphoblastoid. shipped in: dry ice. Storage Class Code: 10 - Combustible liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 12352100
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 940822104-1VL
